IHC of ZAP-70 on an FFPE Tonsil Tissue
| Intended Use | For In Vitro Diagnostic Use | |||||||||||||||||||||||||||||||||||
| Summary and Explanation | ZAP-70 is an abbrevation for Zeta-chain-associated protein kinase 70 (70 is the molecular weight in kDa). The protein is a member of the protein-tyrosine kinase family. ZAP-70 is normally expressed in T-cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 in B-cells is used as a prognostic marker in identifying different forms of Chronic Lymphocytic Leukemia (CLL). ZAP-70 protein is expressed in leukemic cells in approximately 25% of Chronic Lymphocytic Leukemia (CLL) cases as well. ZAP-70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (ZAP-70 negative) and Ig-unmutated (ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The ZAP-70 positive Ig-unmutated CLL cases have a poorer prognosis. | |||||||||||||||||||||||||||||||||||
| Antibody Type | Mouse Polyclonal | Clone | 2F3.2 | |||||||||||||||||||||||||||||||||
| Isotype | IgG2a | Reactivity | Paraffin, Frozen | |||||||||||||||||||||||||||||||||
| Localization | Cytoplasmic | Control | Tonsil, Lymph Node, Chronic Lymphocytic Leukemia | |||||||||||||||||||||||||||||||||
| Presentation | ZAP-70 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. | |||||||||||||||||||||||||||||||||||
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| Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product. | ||||||||||||||||||||||||||||||||||||
