IHC of CK Oscar on FFPE Kidney Tissue (ALK Red)
IHC of CK 34BE12 on FFPE Breast (ALK Red)
IHC of CK OSCAR on FFPE Skin Tissue (ALK Red)
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| Intended Use | For In Vitro Diagnostic Use | |||||||||||||||||||||
| Summary and Explanation | PolyDetector Alk Red Ready-to-Use Substrate-Chromogen is a solution suitable for use in immunohistochemical, immunocytochemical and in situ hybridization procedures using Alkaline Phosphatase Detection Systems. PolyDetector Alk Red Ready-to-Use Substrate-Chromogen allows for the demonstration of tissue antigens or nucleic acids in paraffin-embedded tissues, cryostat sections, cytosmears, and cell preparations. The substrate-chromogen is the final step in the detection portion; it enables the antibody-antigen-enzyme complex (or nucleic-acid-probe-enzyme complex) to be viewed under a light microscope. This occurs when Alk Red, in the presence of Alkaline Phosphatase, gets deposited at the site of the target antigen or nucleic acid, producing a red color. Alk Red is soluble in organic solvents and therefore should be mounted with water-based mounting media, such as Aqua Mounter (BSB 0090-BSB 0093) or can be mounted with permanent medium when using the Bio SB ChromoProtector (BSB 0151 – BSB 0156). | |||||||||||||||||||||
| Presentation | PolyDetector Alk Red Ready-to-Use Substrate-Chromogen (color forming molecule) is a ready-to-use solution that develops into a red precipitate. | |||||||||||||||||||||
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