IHC of Cytokeratin 5, 6 & ERG on an FFPE Breast Tissue
| Intended Use | For In Vitro Diagnostic Use | |||||||||||||||||||||||||||||||||||
| Summary and Explanation | Cytokeratin 5 is a type II cytokeratin found in squamous cell epithelium, myoepithelial cells of the breast and the basal cells of the prostate. Cytokeratin 6 is a type II cytokeratin known for its strong induction in stratified epithelia that features an enhanced cell proliferation rate or abnormal differentiation during wound healing. ERG is expressed at higher levels in early myelocytes than in mature lymphocytes, and thus ERG may act as a regulator of differentation of early hematopoietic cells. ERG can fuse with TMPRSS2 protein to form an oncogenic fusion gene commonly found in prostate cancer. Cytokeratin 5 is expressed in most epithelial mesotheliomas but not by most pulmonary adenocarcinomas and can be used to differentiate between the two. Together, CK 5 & 6 can be used to differeniate Mesothelioma (positive) from Lung Carcinoma (negative) or metastatic carcinoma (negative), and can also be used to distinguish between Ductal Hyperplasia of the breast (positive) from Solid Papillary DCIS (negative). ERG antibody labels prostate cancer cells, endothelial cells, and lymphocyes. | |||||||||||||||||||||||||||||||||||
| Antibody Type | Rabbit Monoclonal | Clone | EP24/EP67/EP111 | |||||||||||||||||||||||||||||||||
| Isotype | IgG | Reactivity | Paraffin, Frozen | |||||||||||||||||||||||||||||||||
| Localization | Cytoplasmic, Nuclear | Control | Breast and Prostate Carcinomas | |||||||||||||||||||||||||||||||||
| Presentation | CK5, CK6 & ERG is a rabbit monoclonal antibody cocktail derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. | |||||||||||||||||||||||||||||||||||
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| Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product. | ||||||||||||||||||||||||||||||||||||
