IHC of Cytokeratin 20 on an FFPE Colon Cancer Metastasis to LungTissue
| Intended Use | For In Vitro Diagnostic Use | |||||||||||||||||||||||||||||||||||
| Summary and Explanation | Cytokeratin 20 (CK 20) is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. Cytokeratin 20 is a Type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells found in the gastric and intestinal mucosa. CK 20 is expressed in Adenocarcinomas of the colon, stomach, pancreas and biliary system. It is also expressed in Mucinous Ovarian Tumors, Transitional-cell Carcinomas of the urinary tract, and Merkel-cell Carcinomas. Cytokeratin 20 is useful in the differentiation of specific types of simple epithelial cells of the urinary tract and normal and malignantly-transformed epithelia. This antibody is essentially non-reactive in Squamous Cell Carcinomas and Adenocarcinomas of the Breast, Lung, and Endometrium, Non-mucinous Tumors of the Ovary, and Small-cell Carcinomas. This antibody is often used in conjunction with CK 7 and other antibodies to distinguish Colon Carcinomas (CK20+) from Ovarian, Pulmonary, and Breast Carcinomas. | |||||||||||||||||||||||||||||||||||
| Antibody Type | Rabbit Monoclonal | Clone | EP23 | |||||||||||||||||||||||||||||||||
| Isotype | IgG2a/K | Reactivity | Paraffin, Frozen | |||||||||||||||||||||||||||||||||
| Localization | Cytoplasmic | Control | Colon Carcinoma, Colon Mucosa, Bladder | |||||||||||||||||||||||||||||||||
| Presentation | CK20 is a Rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. | |||||||||||||||||||||||||||||||||||
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| Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product. | ||||||||||||||||||||||||||||||||||||
