IHC of Collagen Type IV on an FFPE Skin Tissue
| Intended Use | For In Vitro Diagnostic Use | |||||||||||||||||||||||||||||||||||
| Summary and Explanation | Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. Collagen IV is a major constituent of the basement membranes, along with laminins and enactins. It is composed of the alpha 1 IV chain and alpha 2 IV chain in a 2:1 ratio. It can form insoluble fibers with high tensile strength. Normal tissue stains with this antibody in a manner consistent with the sites of mesenchymal elements and epithelial basal laminae. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. Collagen IV can also be useful in the classification of soft tissue tumors; Schwanomas, Leiomyomas, and their well-differentiated malignant counterparts usually immunoreact to this antibody. The vascular nature of neoplasms, Hemangiopericytoma, Angiosarcoma and Epithelioid Hemangioendothelioma can be observed with this antibody. | |||||||||||||||||||||||||||||||||||
| Antibody Type | Mouse Monoclonal | Clone | CIV22 | |||||||||||||||||||||||||||||||||
| Isotype | IgG1/K | Reactivity | Paraffin, Frozen | |||||||||||||||||||||||||||||||||
| Localization | Cytoplasmic | Control | Muscle, Lung | |||||||||||||||||||||||||||||||||
| Presentation | Collagen Type IV is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. | |||||||||||||||||||||||||||||||||||
| Special Note | Collagen Type IV antibody requires the use of ImmunoDNA Digestor (Cat.#’s BSB 0108, BSB 0109, BSB 0110, BSB 0111, BSB 0112) or similar Proteinase K based product as antigen retriever. | |||||||||||||||||||||||||||||||||||
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| Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product. | ||||||||||||||||||||||||||||||||||||
