Actin is a globular-structural, 345 kDa protein that polymerizes in a helical fashion to form an actin filament (or microfilament). Actin filaments provide mechanical support for the cell, determine the cell shape, enable cell movements (through lamellipodia, filopodia, or pseudopodia); and participate in certain cell junctions, in cytoplasmic streaming and in contraction of the cell during cytokinesis. In muscle cells they play an essential role, along with myosin, in muscle contraction. In the cytosol, actin is predominantly bound to ATP, but can also bind to ADP. This antibody recognizes actin of skeletal, cardiac, and smooth-muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are nonreactive. Neoplastic cells of non-muscle-derived tissue such as Carcinomas, Melanomas and Lymphomas are
negative. This antibody is useful in the identification of rhabdoid cellular elements.
Antibody Type
Mouse Monoclonal
Clone
HHF-35
Isotype
IgG1/K
Reactivity
Paraffin, Frozen
Localization
Cytoplasmic
Control
Skeletal Muscle, Appendix, Prostate
Presentation
Actin, Muscle Specific is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.
Antibody Type
Dilution
Volume/QTY
BSB 5022
Prediluted
Ready-To-Use
3.0 ml
BSB 5023
Prediluted
Ready-To-Use
7.0 ml
BSB 5024
Prediluted
Ready-To-Use
15.0 ml
BSB 5025
Concentrated
1:50-1:200
0.1 ml
BSB 5026
Concentrated
1:50-1:200
0.5 ml
BSB 5027
Concentrated
1:50-1:200
1.0 ml
BSB 5028
Control Slides
5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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