IHC of CD117 on an FFPE GIST Tissue
| Intended Use | For In Vitro Diagnostic Use | |||||||||||||||||||||||||||||||||||
| Summary and Explanation | CD117 is a tyrosine-kinase receptor for stem cell factor (SCF), also known as “steel factor” or “c-kit ligand”. C-kit is a polypeptide that activates bone marrow precursors of a number of blood cells, but its receptor is also present in other cells. C-kit mutations in the interstitial cells of Cajal in the digestive tract are probably the key to Gastrointestinal Stromal Tumors (GISTs). CD117 is found on interstitial cells of Cajal, germ cells, bone marrow stem cells, melanocytes, breast epithelium and mast cells. This receptor is found on a wide variety of tumor cells (Follicular and Papillary Carcinoma of the Thyroid, Adenocarcinomas from endometrium, lung, ovary, pancreas, breast; Malignant Melanoma, Endodermal Sinus Tumor, Small-cell Carcinoma) but has been particularly useful in differentiating Gastrointestinal Stromal Tumors (GIST) from Kaposi’s Sarcoma and tumors of smooth-muscle origin. | |||||||||||||||||||||||||||||||||||
| Antibody Type | Rabbit Monoclonal | Clone | EP10 | |||||||||||||||||||||||||||||||||
| Isotype | IgG | Reactivity | Paraffin, Frozen | |||||||||||||||||||||||||||||||||
| Localization | Cytoplasmic, Membranous | Control | Skin, Testis, Breast, GIST, Colon, Brain, Tonsil | |||||||||||||||||||||||||||||||||
| Presentation | CD117 is rabbit monoclonal antibody from supernatant diluted in Phosphate Buffered Saline, pH 7.6, with protein base, and preserved with Sodium Azide preservative. | |||||||||||||||||||||||||||||||||||
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| Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product. | ||||||||||||||||||||||||||||||||||||
| * The CD117 antibody, clone EP10, has been manufactured using Epitomics RabMab® technology covered under Patent No.’s 5,675,063 and 7,402,409. | ||||||||||||||||||||||||||||||||||||
