IHC of MLH1 on an FFPE Colon Carcinoma Tissue
| Intended Use | For In Vitro Diagnostic Use | |||||||||||||||||||||||||||||||||||
| Summary and Explanation | MLH1 is a mismatch repair gene of around 87 kDa, commonly associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC). This gene was identified as a locus frequently mutated in HNPCC. It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. In a high proportion of patients with microsatellite instability (MSI-H), the MLH1 protein is typically deficient. This protein deficiency is linked to the autosomal dominant condition of Hereditary Non-Polyposis Colon Cancer. The anti-MLH1antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite-unstable have a better prognosis than their microsatellite stable counterparts. | |||||||||||||||||||||||||||||||||||
| Antibody Type | Mouse Monoclonal | Clone | G168-728 | |||||||||||||||||||||||||||||||||
| Isotype | IgG2a/K | Reactivity | Paraffin, Frozen | |||||||||||||||||||||||||||||||||
| Localization | Nuclear | Control | Testis, Tonsil, Colon, Kidney, Colon Carcinoma | |||||||||||||||||||||||||||||||||
| Presentation | Anti-MLH1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. | |||||||||||||||||||||||||||||||||||
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| Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product. | ||||||||||||||||||||||||||||||||||||
