The deparaffinization procedure with TintoDeparaffinator Hot Rinse, TintoDeparaffinator Citrate, and TintoDeparaffinator EDTA solutions uses a heat-assisted deparaffinization method while achieving tissue hydration and retrieval of proteins and nucleic acids. Deparaffinization at moderately elevated temperatures has been shown to adequately dissolve paraffin at significantly lower solvent concentrations compared to methods of deparaffinization at ambient temperatures using xylenes and/or xylene substitutes. By utilizing lower non-toxic solvent concentrations, the process of tissue rehydration following deparaffinization and retrieval can be achieved with a single final rinse step to remove micro paraffin leftovers, followed by direct transfer of slides to a buffer bath. These reagents are specifically designed to work with the Bio SB TintoRetriever Pressure Cooker (BSB 7008) or TintoRetriever PT Module (BSB 7030 and BSB 7033) but may be used with any heating device that provides the required temperature.

These reagents are designed to deparaffinize, rehydrate and retrieve one microscope slide containing a standard size (from 0.5cm x 1cm up to 2.5cm x 2.5cm diameter) paraffin-embedded tissue section per 3 ml of TintoDeparaffinator Hot Rinse 1X.  A typical staining dish containing 200-250 ml of the TintoDeparaffinator Hot Rinse 1X solution will help deparaffinize approximately 72 standard tissue sections.  If larger tissue sections are used, the number of processed slides may be less.  If residual paraffin is observed microscopically on the slides following deparaffinization, the deparaffinization reagents should be replaced.

TintoDeparaffinator Hot Rinse is intended to be used as a final rinse step in order to help remove residual micro-paraffin leftover on the slides after the use of heat treatment with either the TintoDeparaffinator Citrate or the TintoDeparaffinator EDTA.

ChromoProtector is highly recommended to be used after the intended staining procedure in order to remove any significant micro-paraffin that may be leftover before mounting slide with a coverslip.