Actin is a major component of the cytoskeleton and is present in every cell type. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms (alpha, beta and gamma) have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Smooth-Muscle Actin antibody does not stain cardiac or skeletal muscle; however, it will stain myofibroblasts and myoepithelial cells. This antibody could be used together with Muscle-Specific Actin to distinguish Leiomyosarcoma from Rhabdomyosarcoma. In most cases of Rhabdomyosarcoma, this antibody gives negative results whereas M. S. Actin is positive in the rhabdomyoblasts. Leiomyosarcomas are positive with both M. S. Actin and S. M. Actin antibodies.
Antibody Type
Mouse Monoclonal
Clone
BSB-15
Isotype
IgG2a/K
Reactivity
Paraffin, Frozen
Localization
Cytoplasmic
Control
Appendix, Uterus
Presentation
S. M. Actin is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.
Antibody Type
Dilution
Volume/QTY
BSB 5029
Prediluted
Ready-To-Use
3.0 ml
BSB 5030
Prediluted
Ready-To-Use
7.0 ml
BSB 5031
Prediluted
Ready-To-Use
15.0 ml
BSB 5032
Concentrated
1:250-1:1000
0.1 ml
BSB 5033
Concentrated
1:250-1:1000
0.5 ml
BSB 5034
Concentrated
1:250-1:1000
1.0 ml
BSB 5035
Control Slides
5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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